Tieteelliset julkaisut

Home-Applied Dual-Light Photodynamic Therapy in the Treatment of Stable Chronic Periodontitis (HOPE-CP)—Three-Month Interim Results

Adjunctive use of Lumoral dual-light antibacterial aPDT in non-surgical periodontal treatment (NSPT) results in improved treatment outcomes at three months post-therapy.

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Repeated Home-Applied Dual-Light Antibacterial Photodynamic Therapy Can Reduce Plaque Burden, Inflammation, and aMMP-8 in Peri-Implant Disease—A Pilot Study

This pilot study tested the benefit of a commercially available Lumoral® device designed for regular periodontal dual-light aPDT treatment at home. Seven patients with peri-implant disease applied dual-light aPDT daily in addition to their normal dental hygiene for four weeks. The results suggest a benefit of regular application of dual-light aPDT in peri-implantitis. Frequently repeated application can be a promising approach to diminishing the microbial burden and to lowering the tissue destructive proteolytic and inflammatory load around dental implants.

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Daily Administered Dual-Light Photodynamic Therapy Provides a Sustained Antibacterial Effect on Staphylococcus aureus

New means to reduce excessive antibiotic use are urgently needed. This study tested dual-light aPDT against Staphylococcus aureus biofilm with different relative ratios of light energy with indocyanine green.

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Dual-Light Photodynamic Therapy Effectively Eliminates Streptococcus Oralis Biofilms

During cancer treatment, oral mucositis due to radiotherapy or chemotherapy often leads to disruption of the oral mucosa, enabling microbes to invade bloodstream. Viridans streptococcal species are part of the healthy oral microbiota but can be frequently isolated from the blood of neutropenic patients. We have previously shown the antibacterial efficacy of dual-light, the combination of antibacterial blue light (aBL) and indocyanine green photodynamic therapy (aPDT).

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Indocyanine Green-Assisted and LED-Light-Activated Antibacterial Photodynamic Therapy Reduces Dental Plaque

Antimicrobial photodynamic therapy (aPDT) has been introduced as an adjunct method for dental hygiene. Although antibacterial and antiplaque effects resulting from aPDT have already been demonstrated in the literature, effects on bacterial flora diversity or early gingivitis biomarkers have not previously been established.

Antimicrobial photodynamic therapy resulted in a significant reduction of plaque formation. An analysis of the 16S rRNA sequencing found a reduction in the Streptococcus, Acinetobacterial, Capnocytophagal, and Rothia bacteria species and a gain in Neisseria and Hemophilus bacteria on the aPDT-treated side. The gain in the latter group of bacteria superseded the relative loss of the former in the plaque, while alpha diversity remained stable. A reduction of the total amount of MMP-8 in the GCF was seen on the treated side, suggesting inhibition of early gingivitis.

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Dual-light photodynamic therapy administered daily provides a sustained antibacterial effect on biofilm and prevents Streptococcus mutans adaptation

Antibacterial photodynamic therapy (aPDT) and antibacterial blue light (aBL) are emerging treatment methods auxiliary to mechanical debridement for periodontitis. In this study, we tested Streptococcus mutans biofilm sensitivity to either aPDT, aBL or their combination dual-light aPDT (simultaneous aPDT and aBL) exposure. In conclusion, when aPDT is administered repeatedly to S. mutans biofilm, a single wavelength-based aBL or aPDT leads to a significant biofilm adaptation and increased S. mutans viability. The combined use of aBL light in synchrony with aPDT arrests the adaptation and provides significantly improved and sustained antibacterial efficacy.

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A Review of Fluorescent Imaging in Surgery

The purpose of this paper is to give an overview of the recent surgical intraoperational applications of ICG fluorescence imaging methods, the basics of the technology, and instrumentation used. Well over 200 papers describing this technique in clinical setting are reviewed. In addition to the surgical applications, other recent medical applications of ICG are briefly examined. Fluorescence Imaging (FI) is one of the most popular imaging modes in biomedical sciences for the visualisation of cells and tissues both in vitro and in vivo.

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Fototerminen terapia ja Lumoral hammaslääketieteessä

Valolla on tiedetty olevan hoidollista vaikutusta jo vuosituhansia, mutta vuonna 1898 Oscar Raab ryhtyi ensimmäisenä lähestymään asiaa tieteellisesti. Hän dokumentoi ensimmäisenä valoaktivaatioon perustuvan antibakteerisen vaikutuksen. Tällöin menetelmän laajaa käyttöönottoa haittasi kuitenkin sopivien valolähteiden puute.

Antibioottien löytyminen bakteeri-infektioiden hoitoon vuonna 1928 korvasi tarpeen valoaktivoitaville menetelmille. Sittemmin on kuitenkin havaittu, että systeemisesti annettavat antibiootit haittaavat laajasti kehon normaalia bakteeritasapainoa. Samaan aikaan antibioottiresistenssistä on muodostunut alati kasvava uhka terveydenhuollolle.

Lokaalissa valoaktivoitavassa hoidossa antibakteerinen vaikutus kohdentuu vain valoaktivoitavaan kohtaan, eikä se vaikuta kehon normaaliflooraan. Valoaktivoitaville menetelmille ei myöskään muodostu antibioottiresistenssiä.

Kuva 2. Vasemmalla: Porphyromonas gingivalis-bakteeriviljelmä verimaljalla. Oikealla: Lumoral-menetelmällä inaktivoitu samanlainen bakteeriviljelmä.